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Our group has long-standing experience in cell-based RNAi screening in Drosophila. We constantly develop and optimize screening reagents and methods. We developed and generated a novel genome-wide Drosophila RNAi library with an optimized design. Several genome-wide RNAi screens have been conducted so far using the second-generation library (Heidelberg 2). This library recently served to generate a genome-wide in vivo RNAi Drosophila library (VDRC KK library), enabling to perform cell-based and in vivo studies using the same RNAi constructs. The library is constantly updated according to new gene annotations and further developed in terms of specificity and efficiency.


Heidelberg 1 (HFA)

This library is the original Drosophila library containing the HFA amplicons as templates that were generated by Renato Paro's laboratory. The library has been largely replaced by newer designs. Complete sequence information can be obtained from Boutros et al. (Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science, 2004) or by browsing the GenomeRNAi database.


Heidelberg 2 (BKN)

The library has been completely redesigned using the software Next-RNAi (Design and evaluation of genome-wide libraries for RNAi screens. Horn et al. Genome Biol., 2010). The constructs have been optimized to exclude low complexity regions and other sequence motifs that induce off-target effects. The library (unpublished) contains all BKN amplicons and is arranged in different formats:
  • Genome-wide library
  • Kinase library containing three amplicons against all kinases in the Drosophila genome
  • Phosphatase library
  • Transcription factor library
  • C. elegans negative control library

 

Enquiries about RNAi reagents

The second-generation Drosophila dsRNA library (Heidelberg 2) can be obtained from us in a pre-aliquoted ready-to-use form. We provide whole-genome RNAi libraries, sub-libraries or custom-made sets for selected targets. Please contact us for costs and further information.

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